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Regulation of matrix metalloproteinase-9 protein expression by 1alpha,25-(OH)2D3 during osteoclast differentiation
Journal of Veterinary Science ; : 133-140, 2014.
Article in English | WPRIM | ID: wpr-56424
ABSTRACT
To investigate 1alpha,25-(OH)2D3 regulation of matrix metalloproteinase-9 (MMP-9) protein expression during osteoclast formation and differentiation, receptor activator of nuclear factor kappaB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were administered to induce the differentiation of RAW264.7 cells into osteoclasts. The cells were incubated with different concentrations of 1alpha,25-(OH)2D3 during culturing, and cell proliferation was measured using the methylthiazol tetrazolium method. Osteoclast formation was confirmed using tartrate-resistant acid phosphatase (TRAP) staining and assessing bone lacunar resorption. MMP-9 protein expression levels were measured with Western blotting. We showed that 1alpha,25-(OH)2D3 inhibited RAW264.7 cell proliferation induced by RANKL and M-CSF, increased the numbers of TRAP-positive osteoclasts and their nuclei, enhanced osteoclast bone resorption, and promoted MMP-9 protein expression in a concentration-dependent manner. These findings indicate that 1alpha,25-(OH)2D3 administered at a physiological relevant concentration promoted osteoclast formation and could regulate osteoclast bone metabolism by increasing MMP-9 protein expression during osteoclast differentiation.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteoclasts / Tetrazolium Salts / Thiazoles / Acid Phosphatase / Calcitriol / Calcium Channel Agonists / Gene Expression Regulation, Enzymologic / Cell Differentiation / Cell Line / Blotting, Western Limits: Animals Language: English Journal: Journal of Veterinary Science Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteoclasts / Tetrazolium Salts / Thiazoles / Acid Phosphatase / Calcitriol / Calcium Channel Agonists / Gene Expression Regulation, Enzymologic / Cell Differentiation / Cell Line / Blotting, Western Limits: Animals Language: English Journal: Journal of Veterinary Science Year: 2014 Type: Article