Expression of DNA methyltransferase 1 in human cervical carcinoma tissue and cells and its significance / 解放军医学杂志

ABSTRACT

Objective To study the expression of DNA methyltransferase 1 (DNMT1) in human cervical carcinoma tissue and cells, analyze the relationship between the expression level of DNMT1 and the clinicopathologic features of cervical carcinoma, and explore the role DNMT1 plays in tumorigenesis of cervical carcinoma. Methods The expression levels of DNMT1 protein in 3 cervical cancer cell lines, 89 samples of cervial cancer tissue, 20 samples of cervical intraepithelial neoplasia (CIN) and 20 samples of normal cervix were determined by immunohistochemistry method. The expression levels of DNMT1 mRNA in all the cell lines and 79 paraffin sections of cervix tissue were determined by RT-PCR. Results The DNMT1 protein was expressed in all the three cervix cancer cell lines. Positive expression was detected in 71.9% of cervix cancer samples, 35.0% of CIN samples and 10.0% of normal cervix samples, with significant differences among the three groups (P