Autophagy of peripheral monocytes following trauma:study on 51 cases / 第三军医大学学报

ABSTRACT

Objective To investigate the change of monocyte autophagy in patients after trauma.Methods ①According to Injury Severity Score(ISS) or Abbreviated Injury Scale(AIS),we divided our 51 subjected trauma patients into 3 groups,the minor injury group(n=21),the moderate trauma group(n=19) and the severe trauma group(n=11).Another 14 healthy volunteers served as control.We collected the peripheral blood in 24 h,3,5 and 10 d after trauma,then harvested the monocytes by density gradient centrifugation with adherence separation.Then we observed the autophagy phenomenon by using laser confocal microscopy and transmission electron microscopy;② After the isolated monocytes were dyed with MDC and lysed,they were detected and quantified for fluorescent intensity by fluorospectrophotometer(excitation wavelength 380 nm and emission wavelength 525 nm);③Blood sugar level of all patients were measured in 24 h,3 and 5 d respectively after trauma,and the correlation between autophagy and blood sugar was analyzed.Results Compared with control group,more autophagy in monocytes was observed and quantified in trauma patients than in health volunteers(P