Expression of NF-?B subunits P50 and c-Rel protein in primary cortical neurons after oxygen glucose deprivation/reoxygenation / 第二军医大学学报

ABSTRACT

Objective:To investigate the expression of nuclear factor-?B(NF-?B)subunits P50 and c-Rel protein in primary cortical neurons of Wistar rats at different time points of oxygen glucose deprivation/reoxygenation(OGD/R).Methods:The neurons dissociated from the cortex of the neonatal rats were primary cultured and were identified by immunocytochemistry.OGD/R model was established.The study was divided into 6 groups according to different processing methods,including normal group,OGD 4 h treated,OGD 4 h/R 2 h treated,OGD 4 h/R 6 h treated,OGD 4 h/R 12 h treated and OGD 4 h/R 24 h treated groups.The expression of NF-?B P50 and c-Rel protein in neurons was examined by immunocytochemistry method and Western blotting.Results:(1)Immunocytochemistry detection targeting neuron specific enolase(NSE)and beta-Ⅲ tubulin confirmed that the cultured cells were neurons.(2)The expression of NF-?B P50 protein was significantly higher in OGD 4 h group than in control group(P