Influence of inhibited ubiquitin proteasome system to heat shock protein 27 and tumor necrosis factor-? in cardiac ischemia-reperfusion rat / 第三军医大学学报

ABSTRACT

Objective To study the influence on heat shock protein 27 (Hsp27) and tumor necrosis factor ? (TNF-?) by inhibiting ubiquitin proteasome system in cardiac ischemia-reperfusion (I/R) rats. Methods Left anterior descending (LAD) coronary artery was ligated for 30 min and then released for 6 h in adult Sprague-Dawley rats. Five minutes before reperfusion, MG-132 (N-benzoyloxycarbonyl (Z)-Leu-Leuleucinal) at dose of 0.75 mg/kg was given by vein injection in I/R+T group, but I/R group, I group and sham group were given the same volume of sterile saline. The changes of myocardial infarct size, cardiac protein and mRNA levels of Hsp27 and TNF-? were measured. Results Compared with I/R group, the myocardial infarct size was significantly reduced (P