Bone morphogenetic protein-4 regulates embryonic tongue morphogenesis / 北京大学学报(医学版)

ABSTRACT

Objective:To analyze the effect of Bone morphogenesis 4 and its antagonist Noggin on morphogenesis of tongue.Methods: Dissected rats to get embryonic day 13(E13) tongues;fed E13 tongues in standard medium,BMP4(0.03 mg/L,0.3 mg/L,1 mg/L),and the antgonist Noggin(1 mg/L,3 mg/L,10 mg/L) medium;cultured for 3 days;fixed samples,observed tongues with scanning electronic microscope(SEM);measured the whole tongue length,anterior 1/8,1/4 width and middle width of cultured tongues and analyzed data with SPSS 10.0.To further study the effects of BMP4 on epithelial and mesenchymal cell proliferation,Affi-gel blue gel beads were applied.Beads were soaked in PBS and BMP4(667 mg/L),and implanted in the E13 embryonic tongues;then after cultured in standard medium for 3 days,tongues were embedded in O.C.T.and cut into 12 ?m series sections.Ki67 was detected by immunohistochemical method.Results:(1)Whole length of tongues changed greatly(P