RELATIONSHIP OF INCREASED INTRACELLULAR S-ADENOSYLHOMOCYSTEINE WITH MONOCYTE CHEMOATTRACTANT PROTEIN -1 EXPRESSION IN HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS / 营养学报
Acta Nutrimenta Sinica
;
(6)1956.
Article
in Chinese
| WPRIM
| ID: wpr-565099
ABSTRACT
Objective To explore the molecular basis of vascular endothelial cell's injury by cellular S-adenosylhomocysteine (SAH) accumulation, and the mechanism for cardiovascular disease induced by dietary high methionine intake. Method After human umbilical vein endothelial cells (HUVEC) were treated without (normal) or with different concentrations of potent S-adenosylhomocysteine hydrolase (SAHH) inhibitor 3-deazaadenosine (DZA) for 24, 48 or 72h, the level of intracellular inflammatory monocyte chemoattractant protein-1 (MCP-1) was measured with enzyme linked immunosorbent assay (ELISA). Then, the relative expression of MCP-1 mRNA was detected with quantitative reverse transcription polymerase chain reaction (qRT-PCR). Furtheremore, the methylated state of MCP-1 gene promoter was analyzed with methylation special PCR (MSP) method. Results The level of intracellular MCP-1 was increased in HUVEC incubated with DZA than normal (P
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Nutrimenta Sinica
Year:
1956
Type:
Article
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