Effect of different caesalpinia sappan extract on mice thymus and proliferating function of T-cells / 中华中医药杂志

ABSTRACT

Objective: To observe the effect of different caesalpinia sappan extract on the Mice thymus tissue, peripheral T-cell proliferation and the production of INF-?. Methods: To make preparation of different caesalpinia sappan extract and give them to the mice, weighing the mice thymus and calculating the thymus index 7 days later. The MTT method was used to measure the proliferation ability of T-cells of the mouse spleen. A double antibody sandwich ELISA was used to detect INF-? content in T-cells culture supernatant. Results: The different caesalpinia sappan extract not only have clear inhibitory effect on the weight of thymus but also have inhibitory effect on the proliferation of T-cells and the production of INF-?. The inhibitory effect of caesalpinia sappan ethanol extract and n-butanol extract on the T-cells is much stronger.