Detection of Sample Preparation about Sialy-LewisA/X on the Surface of Colon Carcinoma Cells by Flow Cytometry / 医学研究杂志
Journal of Medical Research
;
(12)2006.
Article
in Chinese
| WPRIM
| ID: wpr-565367
ABSTRACT
Objective To investigate the effects of variable sample preparation about sialy LewisA/X on the surface of colon carcinoma cells.Methods Human colon carcinoma cell suspension was prepared by using EDTA,trypsin,cell scraper,trypsin-EDTA and trypsin-cell scraper.Cell counts and sialy-LewisA/X on the surface of colon carcinoma cells were detected by flow cytometry.Results Trypsin made fluorescence intensity of sialy-LewisA/X on the surface of colon carcinoma cells become weaken.Fluorescence intensity of sialy-LewisA/X had no significant deviation among EDTA,cell scraper and trypsin-cell scraper.Cell counts were multitude in EDTA group or trypsin-cell scraper group.Conclusion Trypsin-cell scraper is a available approach on preparing cell suspension of adherent cell.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Journal of Medical Research
Year:
2006
Type:
Article
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