Effect of pyrrolidine dithiocarbamate on expression of E-selectin and oxidative stress related enzymes in rats with severe acute pancreatitis induced by L-arginine / 第三军医大学学报

ABSTRACT

Objective To explore the effect of pyrrolidine dithiocarbamate(PDTC) on the expression of E-selectin and the activities of enzymes in oxidative stress in the pancreas of rats with severe acute pancreatitis(SAP).Methods Rat model of ASP was build by intraperitoneal injection of L-arginine(totally 5 g/kg,twice in an interval of 1 h).Thirty-six SD rats were assigned to 6 groups randomly and equally,that is,normal control,SAP,PDTC pretreatment group 1(1 mg/kg) and 2(100 mg/kg),and PDTC treatment group 1(1 mg/kg) and 2(100 mg/kg).PDTC pretreatment was preformed by subcutaneous injection of PDTC 1 h before first injection of L-arginine.For the animals in treatment groups,PDTC was given 3 h after first injection.Normal control was given intraperitoneal injection of normal saline.In 24 h after the first injection of L-arginine,the activity of myeloperoxidase(MPO),lipid peroxide(LPO) and nitric oxide synthase(NOS) were detected.Expressions of E-selectin was detected through inmmunohistochemical method,and activity of NF-?B-DNA binding were detected by electrophoretic mobility shift assay(EMSA).Results In SAP groups and PDTC pretreatment and treatment groups,the expression of E-selectin,activities of NF-?B,MPO,LPO,and inducible NOS(iNOS) were higher,but the activity of constitutive NOS(cNOS) was lower than those in the normal control(P0.05).Conclusion PDTC of 1 mg/kg can alleviate the inflammation in SAP by blocking the translocation of NF-?B,however,the 100 mg/kg PDTC shows little protection in this process.