Mechanism of migration of vascular adventitial fibroblasts enhanced by advanced glycation end-products and the inhibition effect of candesartan / 解放军医学杂志

ABSTRACT

Objective To investigate the mechanism of migration of vascular adventitial fibroblasts(AFs) enhanced by advanced glycation end-products(AGEs) and the inhibition effect of candesartan.Methods Isolated vascular adventitial fibroblast of Sprague-Dawley(SD) rats were cultured.Migratory potential was estimated with Transwell chamber in vitro.Expression of receptor for AGEs(RAGE) and phosphorylated mitogen-activated protein kinase(MAPK) of AFs were determined by RT-PCR and Western blotting.Results AGEs modified human serum albumin(AGE-HAS,concentration ranged from 0 to 300mg/L) up-regulated the expression of RAGE at mRNA and protein levels,peaked at a concentration of 200mg/L(P