Study on microassay for screening butyrylcholinesterase inhibitors / 中国药理学通报
Chinese Pharmacological Bulletin
;
(12)1986.
Article
in Chinese
| WPRIM
| ID: wpr-565635
ABSTRACT
Aim To provide practical microassay for screening butyrylcholinesterase(BChE) inhibitors in drug discovery.Methods The enzyme reaction was optimized in 96-well plates under physiological pH value and temperature by orthogonal matrix method.After the assay conditions were finally selected,3 inhibitors and 115 extracts from Guizhou ethno-drugs were tested.Results A practical microassay for screening BChE inhibitors was successfully constituted by using rat serum as the source of BChE.Conclusion The microassay constituted in this work possess advantages of being practical,rapid,reliable and economical.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Screening study
Language:
Chinese
Journal:
Chinese Pharmacological Bulletin
Year:
1986
Type:
Article
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