Antagonism of Bushen Yiqi Prescription on apoptosis of human first trimester trophoblastic cell by regulating Bcl-2 and XIAP in vitro / 中华中医药杂志

ABSTRACT

Objective:To investigate the effect and mechanism of Bushen Yiqi Prescription on apoptosis of human first trimester trophoblasts.Methods:Isolated from first trimester placental tissues,human trophoblasts cultured in media with 5%, 10%,20%drug-containing serum for a 24,48 or 72h period.Then we measured cell viability by MTT assay,cell DNA content by flow cytomester,cell Caspase-3 enzyme activity by Caspase-3 active assay kit,and,cell Fas,FasL,Bcl-2 and XIAP protein.Results:Absorbency of trophoblast in 490nm wavelength increased greatly after being incubated with drug-containing serum for 24,48,and 72 h(P