Synergistic protective effects of sesamin in combination withvitamin E on nephropathy in rats with metabolic syndrome / 中国临床药理学与治疗学

ABSTRACT

AIM: To study the renoprotective and synergistic effects of sesamin in combination with vitamin E on nephropathy in rats with the metabolic syndrome.METHODS: Nephropathy of metabolic syndrome in rats was induced by high-fat and high-carbohydrate diet.Sesamin(30 mg?kg-1?d-1),sesamin +vitamin E [(15+20) mg?kg-1?d-1),sesamin +vitamin E(30+20) mg?kg-1?d-1] and vitamin E(20 mg?kg-1?d-1) were given to rats with metabolic syndrome at 9th week and lasted for 16 weeks.After 24 weeks,the body weight,left kidney humid weight,blood glucose(BG),blood lipids(BL),systolic blood pressure(SBP), renal function,and indexes of oxygenation and antioxygenation for renal cortex were measured.Pathological changes,collagen deposition and iNOS protein and nitrotyrosine expression of kidney were observed by HE-stain,Masson-stain and immunohistochemical method,respectively.RESULTS:(1)In high-fat and refined-sugar diet group(HFS) the levels of BG,BL,SBP,and MDA,OH and NO2-/NO3-in renal cortex were increased;the activities of T-SOD, CAT and GSH-Px in renal cortex were decreased;the expressions of iNOS protein and nitrotyrosine in kidney were up-regulated;the inflammatory cell infiltration and collagen deposition in renal glomerulus and renal interstitium were severe;the glomerular sclerosis and renal interstitial fibrosis were obvious;the renal function was deteriorated.(2)In sesamin +vitamin E [(30+20) mg?kg-1?d-1] group the decreases in BG,BL,SBP,MDA,OH-and NO2/NO3-in renal cortex were obviously observed;the activities of T-SOD,CAT and GSH-Px in renal cortex were increased;the expressions of iNOS protein and nitrotyrosine in kidney were down-regulated;the inflammatory cell infiltration and collagen deposition in renal glomerulus and renal interstitium were improved;the glomerular sclerosis and renal interstitial fibrosis were reversed;the renal function was ameliorated.The therapeutic effect of sesamin +vitamin E[ (30+20) mg?kg-1?d-1] surpassed that of using sesamin or vitamin E alone(P