Specific antitumor effects of allogeneic dendritic cell/osteosarcoma cell fusion vaccine in rats / 解放军医学杂志

ABSTRACT

Objective To investigate the specific antitumor effects induced by allogeneic dendritic cells (DCs)/osteosarcoma cell fusion vaccine in rats. Methods Fusion vaccine of DCs derived from Wistar rat bone marrow and osteosarcoma cells (UMR106) derived from SD rat was generated by electroporation method, and purified by immunomagnetic beads coated with monoclonal antibody OX62, then cocultured with T lymphocytes derived from SD bone marrow to stimulate the proliferation of the T lymphocytes. The proportion of CD8+ and 44+ cells was determined by flow cytometry, and the anti-tumor activity of cytotoxic T lymphocytes (CTLs) was determined by MTT assay. The SD rat osteosarcoma model was established and active immunotherapy was performed through intradermal injection, and the survival rate of the model rats was observed. Results After cocultured with allogeneic fusion vaccine, the proliferation of T cells increased significantly, and the proportion of CD8+ cells increased from 34.2% to 74.9%, while of CD4+ cells decreased from 59.2% to 19.1% (P