THE EXPERIMENTAL STUDY OF CELL-INDUCED DIFFERENTIATION BY INOSITOL HEXAPHOSPHATE IN HT-29 HUMAN COLON CARCINOMA CELL LINE / 营养学报

ABSTRACT

Objective To investigate the effects and mechanisms of inositol hexaphosphate on diffe-rentiation of HT-29 human colon carcinoma cell line. Method Cells were exposed to various concentrations (control,1.8 mmol/L,3.3 mmol/L) of IP6 for different time (1d,3d,5d). Its effects on the cells were evaluated by detecting follow indices(1) Observing ultrastructure of HT-29 cells by transmission electron microscopy. (2) Detecting alkaline phosphate (ALP) activity to evaluate cell differentiation. (3) Expression of c-Myc mRNA was determined by reverse transcription polymerase chain reaction (RT-PCR). (4) Immunocytochemical stain was used to detect expression of Rb protein. Results (1) After having been treated at 3.3mmol/L concentration,the cell structure changed,and appeared tendency of differentiation,such as karyopyknosis,abundant cellular organs and microvillus increase. (2) The activity of alkaline phosphate increased along with the concentration and time. (3) RT-PCR showed that different concentrations of IP6 decreased the expression of c-Myc mRNA. (4) The immunocytochemical stain showed that different concentrations of IP6 increased expression of Rb protein. Conclusion IP6 can induce differentiation of HT-29 cell by augmenting expression of Rb,decreasing expression of c-Myc and blocking cell cycle.