Effect of propofol on nuclear factor-?B signal pathway in myocardium ischemia/reperfusion injury in rats / 中国药理学通报

ABSTRACT

Aim To explore the molecular mechanisms of propofol in cardioprotection,the activation of NF-?B and NF-?B-regulated inflammatory mediator expression was examined with the model of ischemia/reperfusion in rat heart.Methods Rat myocardium I/R injury was induced by occlusion of the left main coronary artery for 30 min and reperfusion for 2 h.Propofol(3,6,12 mg?kg-1?h-1)was intravenously given at 15 min before ischemia.Heart rate(HR),mean arterial blood pressure(MAP) and pressure-rate index(PRI)were recorded under basal conditions,during the occlusion and at the end of reperfusion,respectively.The translocation of NF-?B in the cardiomyocytes was detected by immunohistochemistry,and NF-?B expression was determined by Western blot.The concentrations of TNF-?,IL-1? in serum and myocardium were evaluated by ELISA and radioimmunoassay,respectively.The cardiac amount of mRNA codifying for intercellular adhesion molecule-1(ICAM-1)and inducible nitric oxide synthase(iNOS) was investigated by RT-PCR and in situ hybridization(ISH),respectively.Results At the end of reperfusion,the cardiac function parameters(HR,MAP,pressure-rate index) in I/R group were lower than those of sham group(P