Repeated ischemic preconditioning attenuates rat myocardial ischemical reperfusion injury through down-regulating TLR4 and decreasing peripheral TNF-? and IL-1? levels / 第三军医大学学报

ABSTRACT

Objective To investigate the protective mechanism of ischemic preconditioning for myocardial ischemic reperfusion injury (MI/RI) rats. Methods Eighteen male Sprague–Dawley (SD) rats were randomly separated into Sham operation,ischemic reperfusion (IR) and ischemic preconditioning (IP) groups (6 rats in each group). Peripheral blood and cardiac muscle were collected after the establishment of the above 3 animal models. ELISA was employed to determine proinflammatory cytokines TNF-? and IL-1? production in sera of these animals. RT-PCR and Western blot analysis was used to assay the transcriptional level and protein level of Toll-like receptor 4 (TLR4) in cardiac muscle tissue with pathological change,respectively. Results Compared with IR group,ischemic preconditioning effectively decreased the expression levels of TNF-? and IL-1? in sera of rats in IP group (P