STUDY ON THE INFLUENCE OF CALCIUM, PHOSPHORUS ON PROLIFERATION, DIFFERENTIATION AND MINERALIZATION OF OSTEOBLASTS IN VITRO / 营养学报

ABSTRACT

Objective To clarify the influence of Ca and P on bone metabolism by investigating theeir effects on proliferation, differentiation and mineralization of osteoblasts (OBs) in vitro. Method OBs were cultured in media with Ca(1, 2,4 mmol/L),P(1, 2,4 mmol/L),CaP 12 (1 mmol/L Ca+2 mmol/L P), and CaP 21 (2 mmol/L Ca+1 mmol/L P). Cell proliferation, activity of alkaline phosphatase (ALP), content of osteopontin (OPN) and collagen type I (Col-I) of OBs, and their corresponding mRNA expression were measured. Results Compared with control group,Ca at 4 mmol/L promoted OBs proliferation significanty (P