Effects of colon carcinoma cells on stimulating canalization of human lymph endothelial cells in vitro / 解放军医学杂志
Article
in Zh
| WPRIM
| ID: wpr-566834
Responsible library:
WPRO
ABSTRACT
Objective To explore the effects of colon carcinoma cells on stimulating canalization of human lymphatic endothelial cells(hLECs)in vitro.Methods hLECs in experiment group were cultured with the supernatant of colon carcinoma cell SW480,and they were cultured in endothelial culture medium in control group.The difference of the 2 groups in the ability of canalization was observed,the changes in cytoskeleton and the expression of Prox1 were detected by immunofluorescence assay,and the expression of integrin ?9 was determined by Western blotting.Results In comparison to the control group,hLECs in experiment group showed stronger ability of canalization,as a copious net-structure appeared on day 7 of cultivation,and the typical tube-structure formed finally on day 14.The number of tube-structure,including lymphatic branches,were greater in hLECs of experiment group(2.93?0.56)than control group(1.56?0.26)from day 6 on(P
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Index:
WPRIM
Language:
Zh
Journal:
Medical Journal of Chinese People's Liberation Army
Year:
1982
Type:
Article