Expression of Toll-interacting protein (Tollip) in appendix during acute appendicitis / 第二军医大学学报

ABSTRACT

Objective To observe the expression of Toll-interacting protein ( Tollip) in appendix and analyze its significance during acute inflammation. Methods Thirty-three patients with acute appendicitis were included in the present study and 6 subjects with non-inflammatory appendixes were taken as controls ( without inflammatory changes). The pulse rate,body temperature ( BT) ,white blood cell ( WBC) count and neutrophil ( NEUT) count were observed one hour before appendectomy. Based on H-E staining and pathological examination,the appendix samples were divided into four groupsnon-inflammatory appendix ( A) ,simple appendicitis ( B) ,suppurative appendicitis ( C) and gangrenous appendicitis ( D). The expression of Tollip protein ( the localization,qualitative and semiquantitative analysis) was analyzed using immunohistochemistry and digital image analysis. The correlation of Tollip with pusle rate,BT,WBC and NEUT was also analyzed. Results Significant differences in BT,WBC and NEUT were found between different pathological groups ( P