Effects of gastrin on NF-?B signaling pathway in human colon cancer cells / 中国药理学通报

ABSTRACT

Aim To investigate the effects of gastrin-17 on NF-?B signaling pathway in human colon cancer cells-Colo320WT. Methods All subjects were divided into four groups control, gastrin-17,gastrin-17 + L365,260 ( gastrin-17 receptor blocker) and L365,260 group. Concerning gastrin-17 group,Colo320WT cells were treated by gastrin-17 for 12 h. Concerning gastrin-17+L360,265 group,Colo320WT cells were pretreated by L365,260 for 30 min and then were treated by gastrin-17 for 12 h. Concerning L365,260 group,Colo320WT cells were pretreated by L365,260 for 30 min. NF-?B DNA binding activity was determined by electrophoretic mobility shift assay (EMSA). Expressions of uPA protein and mRNA were assayed by Western blot and RT-PCR,respectively. Results NF-?B DNA binding activity increased more greatly in the Co-lo320WT cells stimulated by gastrin than that of the control group. And the expressions of uPA protein and mRNA were up-regulated more markedly than those of the control group. The above responses resulting from gastrin-17 were partly blocked by gastrin-17 receptor blocker,L365,260. Conclusion Gastrin-17 may activate NF-?B signaling pathway in human colonic cancer cells Colo320WT.