Fingerprints establishment of Rhizoma Atractylodis Macrocephalae by high performance liquid chromatography and analysis of plant resource / 中华中医药杂志
China Journal of Traditional Chinese Medicine and Pharmacy
;
(12)2005.
Article
in Chinese
| WPRIM
| ID: wpr-567166
ABSTRACT
Objective:
To establish the fingerprints of Rhizoma Atractylodis macrocephalae from different origins with high performance liquid chromatography(HPLC)technology,which was furthermore applied in the quality comparison of plant resource.Methods:
In this method,C1 8column(4.6mm?250 mm)was used with the mobile phase containing acetonitrile-water for gradient elution,acetonitrile(A)0-15min,75%;15-20min,75%-95%;20-35min,95%;35-40min,95%-75%;40-50min,75%,flow rate 1 mL/min and wave length 220nm.Results:
The HPLC fingerprints of Rhizoma Atractylodis macrocephalae from different origins were established and the correlated coefficients of each were calculated.9 common peaks were determined in HPLC chromatogram,three of them were identified as atractylenolideⅢ,Ⅰand atractylon respectively.Conclusion:
The method was simple,reproducible and can be used as plant resource selection and quality control of Rhizoma Atractylodis macrocephalae.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Journal of Traditional Chinese Medicine and Pharmacy
Year:
2005
Type:
Article
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