Early skin changes and serum inflammatory mediators in diabetic rats / 第三军医大学学报

ABSTRACT

Objective To observe the histopathological features of early skin lesions and serum inflammatory mediators changes in the diabetes mellitus(DM) rats.Methods A total of 50 Wistar rats at 8 to 10 weeks of age were divided into normal control group(C group,n=25) and diabetic group(DC group,n=25).The rats of DC group were given a high-glucose and high-fat diet for 2 weeks to induce insulin resistance and then an intraperitoneal injection of 35 mg/kg streptozotocin.All 25 rats were identified to be diabetic models with their fasting blood glucose over ≥7.0 mmol/L.Four rats of C group or DC group were killed to take the skin tissues in the 4th,8th and 12th weekend.In the 16th week,the heart blood samples of the left 13 rats of C group and 10 rats of DC group were collected to measure TNF-?,IL-6 and C-RP levels,and the skin samples were also taken for pathological observation and full-thickness and dermal thickness measurement.Results In the 16th week,the TNF-?,IL-6 and C-RP levels of DC group were significantly higher than the normal group(P