Correlation of CT perfusion imaging of rat breast cancer with tumor pathological grade and microvascular density / 第三军医大学学报

ABSTRACT

Objective To study the correlation of CT perfusion imaging ( CTPI) of induced rat breast cancer with tumor pathological grade and microvascular density ( MVD) ,and the diagnostic value of CTPI for breast cancer. Methods Breast cancer was induced in 35 Wister male rats with intragastric 7,12-dimethyl-1,2-benzanthracene ( DMBA) treatment. Routine breast CT scanning and CTPI were performed. After processed at a workstation,CTPI parameters were obtained including blood flow ( BF) ,blood volume ( BV) ,mean transit time ( MTT) ,permeability surface ( PS) ,and time density curve ( TDC). The rats were sacrificed and breast tumor tisue samples were collected. Breast tumor tisue samples were stained with HE and factor Ⅷ-related antigen immunohistochemistry to observe the pathological type and MVD of tumor. Results Breast tumor was successfully induced in 30 rats ( including 8 with breast hyperplasia and 22 with breast cancer). The BF and BV were higher in rats with breast cancer than in those with breast hyperplasia 151. 72 ? 93. 43 vs 42. 96 ? 32. 42 ml/( min.100 g) ,9. 55 ? 7. 88 vs 2. 17 ? 1. 36 ml/100 g,P 0. 05). The positive correlation of MVD with BF,BV and PS was increased with the decreased tumor differentiation with a correlation coefficient of 0. 701,0. 441 and 0. 521,respectively ( P