Effect of Astragalus Membranaceus and Angelica Sinensis on plasminogen activator inhibitor-1 in cultured endothelial cells / 第三军医大学学报
Journal of Third Military Medical University
;
(24)2003.
Article
in Chinese
| WPRIM
| ID: wpr-567770
ABSTRACT
Objective To investigate the effect of Astragalus Membranaceus( Ast) and Angelica Sinensis( Ang) on antithrombosis and explore the molecular mechanism. Methods Plasminogen activator inhibitor-1 ( PAI-1) expression and activity were selected to observe the antithrombosis effect of Ast and Ang ( 3 and 6 mg/ml) on 0. 5 ng/ml TGF-?1 treated endothelial cells. PAI-1mRNA expression was detected by RT-PCR,PAI-1 antigen assay was detected by specific enzyme-linked immunosorbent assays( ELISA) ,and PAI-1 activity was measured by amidolytical assay. Results Ast and Ang significantly inhibited PAI-1 mRNA expreesion,antigen and activity in endothelial cells. The more powerful effects could be observed when treated by Ast and Ang together. Conclusion The PAI-1 mRNA expression,antigen and activity is inhibited effectively by Ast and Ang,which may be the mechanism of their treatment for antithrombosis.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Third Military Medical University
Year:
2003
Type:
Article
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