Effect of Yukuiqing on expression of connective tissue growth factor in human real mesangial cells incubated with AGEs / 中华中医药杂志
China Journal of Traditional Chinese Medicine and Pharmacy
;
(12)2005.
Article
in Chinese
| WPRIM
| ID: wpr-567876
ABSTRACT
Objective:
To investigate the effects of Yukuiqing (YKQ) on the expression of connective tissue growth factor (CTGF) in cultured human renal mesangial cells (HRMC) incubated with AGEs.Methods:
The HRMCs were incubated with AGEs (200 ?g/mL) and 1.25% YKQ which was prepared by Chinese herbal medicine serum pharmacological approach for 0,8,16,24,48 and 72h or different concentrations (0.313%,0.625% and 1.25%) of YKQ for 48h,respectively,which were incubated with DMEM and rat serum (RS) as the control. CTGF mRNA and protein were detected by reverse transcription polymerase chain reaction (RT-PCR) and Western Blotting respectively.Results:
In Yukuiqing group,the expression of CTGF mRNA and protein decreased significantly compared with control groups (P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Journal of Traditional Chinese Medicine and Pharmacy
Year:
2005
Type:
Article
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