Anti-tumor activity of emodin on human pancreatic cancer cell line BXPC-3 in vivo / 中华中医药杂志
China Journal of Traditional Chinese Medicine and Pharmacy
;
(12)2005.
Article
in Chinese
| WPRIM
| ID: wpr-568016
ABSTRACT
Objective:
To investigate the effect and mechanism of emodin (EMO) on human pancreatic cancer cell line BxPC-3 in vivo.Methods:
After the pancreatic cancer model in nude mice was established, the mice were divided into four groups control group (NS 0.2mL/d by i.p. injection), EMO group (EMO 40mg?kg-1?d-1 by i.p. injection), and Gemcitabine (GEM) group (GEM 80mg/kg, twice/week by i.p. injection) with 8 mice each group. After 2 weeks of administration, the mice were sacrificed, detected the body-weight change of nude nice before and after the experiment, and recorded the growth inhibition rate of tumor (TGI). Immunohistochemical (IHC) staining for ki-67 and terminal deoxynucleotidyl transferase-mediated nicked labeling assay (TUNEL) were undertaken to detect the cell proliferation and cell apoptosis in tumor tissue in xenograft nude mice.Results:
The inter-group comparisons in body-weight of nude mice showed no significant difference in comparing group NS(27.0?1.64)g with group EMO(25.1?1.58)g and GEM(25.6? 1.47)g.The EMO group was 38.46%, the GEM group was 44.23%. The inter-group comparisons in immunohistochemical analysis of ki-67 showed significant difference in comparing group NS IOD(219.5?17.98) with group EMO IOD(146.6? 11.57)and GEM IOD(139.5?12.55), (P
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Journal of Traditional Chinese Medicine and Pharmacy
Year:
2005
Type:
Article
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