Immunoregulatory effects of ulinastatin combined with thymic peptide ?_1 on septic patients / 解放军医学杂志

ABSTRACT

Objective To explore the influence of ulinastatin combined with thymic peptide?1on the polarization of T helper(Th) cells,and the immunoregulatory effects on septic patients.Methods Clinical data of 60septic patients admitted to intensive care unit (ICU)of Guangzhou General Hospital of Guangzhou Command from Jun.2006to Oct.2007were collected and randomly divided into treatment group(n=30)and control group(n=30).The control group received classic SSC treatment,while the treatment group was given classical SSC treatment plus ulinastatin and thymic peptide?1.The control group was divided again into death subgroup and survival subgroup according to the living state on day 28.Peripheral blood samples were collected at day 1(before administration)and day 10. CD4+ T cell subset was detected by flow cytometry,and the levels of IFN-?(T helper type-1cytokine)and IL-4(T helper type-2 cytokine)were evaluated semi-quantitatively.Ten healthy volunteers were chosen in the meantime as healthy controls.Results The positive rates of IFN-?and IL-4of CD4+ T cells declined significantly at day 10in treatment group compared with those in control group and before treatment(P0.05).The IFN-?positive rate presented a low level and showed a downward trend after treatment in survival subgroup,while presented a high level and also showed a downward trend after treatment in death subgroup.The IL-4positive rate showed a downward trend in survival subgroup and upward trend in death subgroup.Conclusion Ulinastatin combined with thymic peptide?1may improve the proportion imbalance of IFN-?and IL-4of septic patients,and regulate the immune status.