HISTOCHEMICAL STUDY OF THE EXUDATE CELLS DURING EXPERIMENTAL PERITONITIS IN RABBITS / 解剖学报

ABSTRACT

Acute non-specific peritonitis was induced by injecting turpentine into rabbits' peritoneal cavity. The exudate cells were collected 1, 3, 8, and 14 days after the injection from 50 experimental and 10 normal rabbits. Normal peritoneal exudate consists of 87.1% small lymphocytes, 9.7% macrophages and small amount of polymorphonuclear granulocytes (PMG). One day after injection the number of PMG increased and fell to normal level 3 days after injection. The number of macrophages gradually increased and reached the peak value 14 days after injection and did not fall to the normal level 30 days after injection. Lymphocytes began to increase in number 8 days after injection and did not return to normal 14 days after injection. Histochemical studies showed that A1P of PMG exhibited strong positive reaction one day after injection and the reaction was weakened 3 days after injection; AcP of macrophages showed increasing activity 3 days after injection which reached maximum on the 5th day and began to decrease after the 8th day. PMG and macrophages were positive for fat staining. Macrophages showed maximal fat reaction 3 days after injection, but contained many vesicles negative to fat stain. Percentages of positively ANAE reactive T lymphocytes raised from 2% to 19.5% 5 days after injection. The above results show that during acute non-specific peritonitis induced by turpentine the changes of exudate cell components and histochemical activity may signify that they play a role in immunological function during inflammation.