IMMUNOHISTOCHEMICAL INVESTIGATION OF THE CHANGES OF PANCREATIC ISLET CELLS DURING LIVER REGENERATION FOLLOWING PARTIAL HEPATECTOMY / 解剖学报

ABSTRACT

50 adult albino rats were divided randomly into 3 groups, i, e. partial hepatectomy group, sham-operation group and normal control group. Specimens of tail portion of pancreas were taken at 1, 2, 3, 6 and 14 days after operation respectively. Paraffin sections were made and stained with immunohistochemical PAP method to demonstrate insulin-containing B cells,C-peptide-containing B cells,glucagon-containing A cells and somatostatin-containing .D cells. The changes in hormone secretion activity of B, A and D cells have been observed during liver regeneration period. Based on the different amount of immunoreactive product, the endocrine activity of islet cells can be divided into 3 grades +, ++, and +++. Percentage of each grade of islet cells was calculated and taken as the parameter of endocrine activity of islet cells. Insulin release activity of B cells increased after 1-3 days of operation, and insulin biosynthesis and/or storage enhanced 3-6 days after. Glucagon release activity of A cells raised gradually during 1-3 days after operation, and somatostatin released activity of D cells declined at the same time. After 6 days, endocrine activity of A cells returned to normal level, but release of somatostatin of D cells elevated. Endocrine activity of islet cells all recovered to normal level 14 days later. The changes of endocrine activity of B cells and A cells were closely related to and parallel with regeneration process of liver. The changes of D cells suggest that it probably be a reflection of regulation action on endocrine activities of B cells and A cells.