FINE STRUCTURE OF MMC-1 MOUSE MACROPHAGE-LIKE CELL LINE / 解剖学报

ABSTRACT

The fine structure of MMC-1 mouse macrophage-like cells was studied by means of electron microscopy. Electron micrographs of these macrophages revealed dark cells, light cells and empty cells according to their density. These three cell types could also be found in other cultural cell lines.The dark cells had the highest electron density. They were round, elliptical or spindle-like in shape and many protrusions were observed on the cell surface. The endoplasm and ectoplasm could be divided clearly. There Were no organelles in the latter. In the endoplasm mitochondria, rough endoplasmic reticulum and polyribosome were abundant, lysosomes, Golgi complexes and microfilaments were easily found. Nucleus with irregular shape located in one side of the cell, euchromatins were prominent, and nucleoli appeared in some cells. Light cells had less cytoplasmic processes, dilated mitochondria, abundant lysosomes, fat droplets and dilated nuclei. Empty cells had only a little cytoplasmic processes, less endoplasmic reticulum and a lot of primary and secondary lysosomes. Sparse chromatins were distributed in the dilated nucleus.According to these fine structures and the origin of this cell line the name of the macrophage-like cell line was suggested.