PREPARATION OF ~(35)S-LABEJJED CPS_1 cDNA PROBE FOR IN SITU HYBRIDIZATION / 解剖学报
Acta Anatomica Sinica
;
(6)1989.
Article
in Chinese
| WPRIM
| ID: wpr-568848
ABSTRACT
The carbamyl phosphate synthetase I (CPS,) cDNA probe labelled with alpha ~(35)S-dATP was obtained by using modified nick translation technigue. The specific radioactivity of the obtained probe reached 0.9-1.5?10~8cpm/ug DNA. This probe could be satisfactorily used in RNA-DNA in situ hybrioization for detecting the gene expression of CPS_1 in liver tissue sections. The advantages of using ~(35)S instead of ~(32)P and ~3H for labelling DNA probe were discussed.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Anatomica Sinica
Year:
1989
Type:
Article
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