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ULTRASTRUCTURAL OBSERVATIONS OF THE ENZYMATICALLY DIGESTED HUMAN AMNIOTIC BASEMENT MEMBRANE / 解剖学报
Acta Anatomica Sinica ; (6)1955.
Article in Chinese | WPRIM | ID: wpr-568925
ABSTRACT
Using enzymatic digestions on human amniotic basement membrane (HABM), it was observed ultrastructurally that heparinasedigestion resulted in formation of microvilli on the surface of HABM and thinning and vesiculation of lamina densa and lamina lucida layers. This suggested that the heparinase Ⅱ-digested HABM had increased its surface area for more efficient cell attachment. The main action of heparinase Ⅱ is to digest heparan sulphate on HABM surface, which leads to exposure of more sites of laminin for cell attachment and neurite outgrowth; therefore heparinase Ⅱ-digested HABM can increase neuronal growth to 150%. For those HABM digested by heparinase Ⅰ, collagenase Ⅰ, and chondroitinase AC, they lost most of the sites for attachment and neurite outgrowth and as a consequence, the growth of neurons decreased. This morphological study supports heparinase Ⅱ digested HABM as an ideal substratum for neuronal attachment and growth.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Acta Anatomica Sinica Year: 1955 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Acta Anatomica Sinica Year: 1955 Type: Article