ULTRASTRUCTURAL OBSERVATIONS OF THE ENZYMATICALLY DIGESTED HUMAN AMNIOTIC BASEMENT MEMBRANE / 解剖学报
Acta Anatomica Sinica
;
(6)1955.
Article
in Chinese
| WPRIM
| ID: wpr-568925
ABSTRACT
Using enzymatic digestions on human amniotic basement membrane (HABM), it was observed ultrastructurally that heparinase Ⅱ digestion resulted in formation of microvilli on the surface of HABM and thinning and vesiculation of lamina densa and lamina lucida layers. This suggested that the heparinase Ⅱ-digested HABM had increased its surface area for more efficient cell attachment. The main action of heparinase Ⅱ is to digest heparan sulphate on HABM surface, which leads to exposure of more sites of laminin for cell attachment and neurite outgrowth; therefore heparinase Ⅱ-digested HABM can increase neuronal growth to 150%. For those HABM digested by heparinase Ⅰ, collagenase Ⅰ, and chondroitinase AC, they lost most of the sites for attachment and neurite outgrowth and as a consequence, the growth of neurons decreased. This morphological study supports heparinase Ⅱ digested HABM as an ideal substratum for neuronal attachment and growth.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Anatomica Sinica
Year:
1955
Type:
Article
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