IDENTIFICATION OF SEROTONIN-LIKE IMMUNOREACTIVE NEURONS IN NEURONAL CULTURES FROM E8 CHICK EMBRYO FOREBRAIN / 解剖学报
Acta Anatomica Sinica
;
(6)1957.
Article
in Chinese
| WPRIM
| ID: wpr-568944
ABSTRACT
The expression of serotonin-like immunoreactivity in day 4 neuronal cultures from E8 chick embryo forebrains was investigated by PAP immunocytochemical method. It was found that 98% of the dissociated forebrain cells cultured on laminin substratum were neuron specific enolase immunoreactive positive; and 10.4% of these neurons were mainly multipolar or bipolar and serotonin immunoreactive positive. In order to verify whether the remaining 2% NSE negative forebrain cells were neuroglial cells, we had used anti-glial fibrillary acid protein antibody to show that only 2% of the cultured forebrain cells were composed of GFAP immunoreactive positive cells, i. e. neuroglial cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Acta Anatomica Sinica
Year:
1957
Type:
Article
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