THE NATURE OF MAST CELLS IN HUMAN AND MOUSE SKIN——A DOUBLE IMMUNOENZYMATIC AND IMMUNOFLOURESCENT STAINING STUDY / 解剖学报

ABSTRACT

This experiment was carried out to demonstrate substance P (SP) and VIP in mast cells in human skin by double immunoenzymatic staining, and to examine SP, VIP and serotonin in mast cells in mouse skin by dual immunoflourescence method. The results were as follows1. There were three types of mast cells in human skin by double immunoenzymatic staining i. e. SP immunoreactive mast cells, VIP immunoreactive mast cells and mast cells with coexistence of SP and VIP. In comparison with toluidine blue staining, 8.32％ of mast cells were VIP immunoreactive; 14.51％ were SP immnuoreactive; 7.71％ were SP and VIP coexistence cells.2. Similar to human skin, SP, VIP immunoreactive mast cells and mast cells with coexistence of SP and VIP were found in mouse skin by dual immunoflourescence. They were 12.7％, 7.9％, and 10.3% of she toluidine bleu positive mast cells, respectively, which acounted to about 24.6％ of the toluidine blue positive mast cells.The possible significance of these results were discussed.