THE PROJECTION OF THE TYROSINE-HYDROXYLASE (TH), NEUROTENSIN (NT), CHOLECYSTOKININ (CCK) CONTAINING NEURONS IN THE VENTROMEDIAL MEDULLA TO THE NUCLEUS ACCUMBENS IN THE RAT——A STUDY BY COMBINED HRP RETROGRADE TRACING AND IMMUNOCYTOCHEMICAL METHOD / 解剖学报

ABSTRACT

By using of combined HRP retrograde tracing and immunocytoche mistry methods, the projection from the caudal part of the ventrolateral medulla (VLM) to the nucleus accumbens (Acb) was examined. When HRP was injected into the ventral and medial area of the caudal part of Acb, the labeled cell bodies were mostly found in the bilateral (predominantly ipsilateral) caudal part of the VLM. When HRP technique (HRP injected into the Acb)was combined with immunocytochemical method, many HRP-TH, HRP-NT and HRP-CCK double labeled cell bodies were found in the VLM. The number of the HRP-TH double labeled cell bodies were numerous, while HRP-NT and HRP-CCK doublelabeled cells were less. HRP-TH double labeled neurons were also found in the reticular formation between the solitary tract nucleus and VLM.