Studies on Effective Compositions of Pinecone Ⅲ. Determination of Polysaccharides in Cone of Chinese Pine (Pinus tabulaeformis) / 中草药

ABSTRACT

Quantitative determination of polysaccharides in Pine cones by phenol-sulfuric acid mehod was studied. and the effects of concentration of phenol-sulfuric acid and reaction temperature on color formation were investigated. It was found that the absorbance is linearly correlated to polysaceharide concentrations between 10～78?g/ml (r=0.9999). The analytical recovery was 99.8%, CV% was 1.2% and the minimal detectable concentration was 5?g/ml.