High Performance Liquid Chromatographic Assay of Tetramethylpyrazine in“Fubidou Granule” / 中草药

ABSTRACT

Fubidou Grandle is a new Chinese herbal preparation. It consists of Rizoma Chuanxiong, Fructus Forsythiae and Herba Asari. It is used for the treatment of acute and subacute paran asal sinusitis. This work reports a new HPLC method for the determination of tetramethylpyr azine in the chinese herbal preparation.A revers phase column C18 was used for the separati on with methanol-water (52∶ 48)at 1 .0ml/min as eluentand measured by UV detector at 292nm.The extraction recovery was 99.2％, RSD was 1.1％(n = 4) and the seusitivity limit of quantit ative analysis was 0.5?g/ml. The assayis simple, rapid and sensitive with good reproduction