Tissue Culture of Thickstemen Gentian(Gentiana crassicaulis)and Plant Regeneration Through Somatic Embryogenesis / 中草药
Chinese Traditional and Herbal Drugs
;
(24)1994.
Article
in Chinese
| WPRIM
| ID: wpr-569447
ABSTRACT
Callus of Gentiana crassicaulis was initiated from hypocotyl and cotyledon on MS medium supplemented with 2mg/L 2.4-D and 0.5 mg/L BA.Induction frequencies of callus were 100%.Somatic embryos were formed on MS medium containing 2mg/L BA,3mg/L ZT, 1mg/L NAA,3mg/L GA,three time MS organics and vitamins,6% sucrose and 500 mg/L LH and three time FeSO4 (Na2EDTA) .40% Callus produced somatic embryos.It developed into complete plants on hormon-free MS medium.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
1994
Type:
Article
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