Study on Immobilization Plant Cell Culture and Release of Intracellular Metabolites / 中草药

ABSTRACT

Membrane immobilization of Catharathus roseus (L. )G. Don cell culture was stuudied. Results showed that the production capacity can attain a higher level when the cell layer was 7~8mm thick. Stainless-steel screen(80?m)was suitable for immobllization of C. roses cell. Pressure pulse can enhance the release of intracelluler metabolites. A method to determine allowable cell layer thickness was developed. The calculated value agreed well with experimental results.