Tissue Culture of Chuanxizhangyacai(Swertia mussotii)Ⅰ.Induction and Maintenance of the Callus / 中草药

ABSTRACT

Calli were induced from young leaves,immature seeds and hypocotyl of Swertia mussotii Franch.Three kinds of media,MS,B5 and N6 were set up in the experiment. Calli were established with the highest frequency on to MS medium cantaining 3mg/L 2,4-D and 0. 6mg/L KT.The induction antmaintenence of the calli were affected markedly by the basic media and compound 2,4-D and KT in the media which proved that asextual reproduction of S. mussotii is possible by tissue culture