Determination of Free Lysine in Queen Bee's Embryo Preparation by Spectrophotometry / 中草药
Chinese Traditional and Herbal Drugs
; (24)1994.
Article
in Zh
| WPRIM
| ID: wpr-569580
Responsible library:
WPRO
ABSTRACT
Free lysine in a preparation of queen bee's embryo was isolated using cation exchange resin- The Q-aminoand carboxyl group of lysine was complexed with cupric ion and the E-amino group reacted with FDNB(Fluoro-dinitrobenzene). After acid treatment and extraction with ether,the absorbance of the solution at 395nm was determined by spectrophotometry. Variation coefficient of the determination was less than 6% and the recovery of lysine was 95 % ~ 110 %.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Traditional and Herbal Drugs
Year:
1994
Type:
Article