Assay of Gallic Acid in Urocalum Capsule by RP- HPLC / 中药新药与临床药理
Traditional Chinese Drug Research & Clinical Pharmacology
;
(6)1993.
Article
in Chinese
| WPRIM
| ID: wpr-569624
ABSTRACT
Objective:
A method for the determination of gallic acid in the extract of Quercus salece- na Blume (Fagaceae) and its preparation (Urocalum Capsules) by means of RP-HPLC was established.Method:
The hydrolysable tannins contained in the sample is hydrolyzed with hy- drochloric acid, the hydrolysis component-gallic acid is determined by HPLC using RP-C18 column, 0.005mol. L-1 of phosphoric acid-methanol (98 2) as mobile phase , 270 nm as detec- tion wavelength.Result:
The linear range of gallic acid is 0. 3~3. 1?g , r=0. 9999, the average recovery is 100. 4%.Conclusion:
It is confirmed that the established method for assay of gallic acid meet the criteria of quantitative analysis of herbal medicine by validation of hydrolysis condition, repeatebility, stability and recovery, The method is simple , good reproducible and precise , and it can also be applied to analysis of other herbal medicine containing hydrolysable tannins.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Traditional Chinese Drug Research & Clinical Pharmacology
Year:
1993
Type:
Article
Similar
MEDLINE
...
LILACS
LIS