Determination of Protocatechuic Acid in Xueniao Capsules by TLC-scanning / 中成药

ABSTRACT

Objective: To establish a quantitative method for determination of protocatechuic acid in Xueniao Capsules. Methods: The capsules were extracted with alcohol acetic acid (10∶1) and then solved with absolute alcohol. The sample was applied on a silica H plate, developed and separated in the solution of chloroform acetone methanol acetic acid (7∶2∶1.5∶0.5). The separated spots were determined by TLC scanning at 530nm(? s). Results: The average recovery was 101.4%. Conclusion: This method for determination of protocatechuic acid in Xueniao Capsules was sensitive, accurate and specific. It could be used as quality control for Xueniao Capsules.