EFFECTION OF GnRH ANALOGUE ON INTRACELLULAR CALCIUM MOBILIZATION IN CULTURED RAT STOMACH SMOOTH MUSCLE CELLS / 解剖学报
Acta Anatomica Sinica
;
(6)1989.
Article
in Chinese
| WPRIM
| ID: wpr-569707
ABSTRACT
Objedctive To study the mechanism of GnRH agalogus(Alarelin)on[ Ca2 + ]; mobilization in stomach smooth muscle cells (SSMc)of rats. Methods Cells were cutured and loaded with Fluo-3-AM. [Ca2 + ]; was measured by nuomeent intensity(FL) in each cell with confocal micoopy. Results (1 ) In Hanks solution. l0-7, l0-6, l0 - 5mol L- 1 Alarelin can elevate[ Ca2 + ], its peak - resting values reached 6. 00 ?0. 50 .9. 23 ?0. 62. l8. 97 ?2. 42 respectively, which indicate that the level of [Ca2+ ]; act in an deede- pendent and time - dependent. (2) thntredly. when Alarelin was 10 4mol?L-1, its peak - resting value only reached 6. 32 ?0 .67, conpared with Alarelin l0 5m.l L-1 which was sigificantly lower(P 0. 05 ). (5 )When pertreatment with Lacidipine in Hanks solution, the effect of Alarelin l0 5mol L-1.as partly inhibited(P
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Anatomica Sinica
Year:
1989
Type:
Article
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