Expression of estrogen receptor ? mRNA in endometrial carcinoma and its significance / 中华妇产科杂志

ABSTRACT

Objective To investigate the expression of estrogen receptor ? (ER? ) mRNA and its significance in endometrial carcinoma. Methods Thirty six normal and 45 cancer tissue samples of endometrium were obtained, and the RNA was isolated from the tissues by the acid guanidium thiocyanate phenol chloroform extraction method. Reverse transcription polymerase chain reaction (RT PCR) was used to amplify the ER? exon 5 wild type mRNA (ER? WT) and splicing variant mRNA (ER? E5SV). Electrophoresis of polymerase chain reaction product was performed and the ratio of ER? E5SV/ER? WT was calculated. The sequencing of ER? E5SV and ER? WT was carried out by express DNA sequencer. Results ER? E5SV and ER? WT were both expressed in normal endometrium and endometrial cancer tissues. A region of 139 bp from base pair 812 to 950 (the hormone binding domain) was found to be deleted in ER? E5SV sequence as compared to ER? WT. The quantity of ER? E5SV was more than that of ER? WT in every normal endometrium, and was less in every endometrial cancer, the ratio of ER? E5SV/ER? WT was 2 47?0 99 in normal endometrium, and 0 55?0 12 in endometrial cancer ( P