Effect of hypoxia ischemia on cysteinyl aspartate specific proteinases activity in cerebral tissue of neonatal rat / 中华妇产科杂志

ABSTRACT

Objective To study the effect of hypoxia ischemia on cysteinyl aspartate specific proteinases (caspase 3) activity in cerebral tissue of neonatal rat and probe into its significance Methods To induce hypoxic ischemic brain damage (HIBD), the left carotid artery of rats at day 7 was ligated and animals were exposed to 8% oxygen for 2 hours 0 5,12,24,and 48 hours after HIBD, both ipsilateral and contralateral cerebral tissue were ditected and homogenized caspase 3 activity was measured by cleavage of the colorimetric substrate DEVD pNA Results Caspase 3 activity in ipsilateral cerebral tissue increased gradually after HIBD and peaked at 24 hours, and then decreased significantly at 48 hours( P 0 05) Conclusions Significant activation of caspase 3 after cerebral hypoxia ischemia strongly suggests that apoptosis is involved in HIBD Application of caspase inhibitors or other anti apoptotic agents may become a new therapeutics of HIBD