RP-HPLC determination of daidzein, genistein, daidzin and genistin in soybean extract / 中草药
Chinese Traditional and Herbal Drugs
;
(24)1994.
Article
in Chinese
| WPRIM
| ID: wpr-569743
ABSTRACT
Object A method for the determination of daidzein, genistein, daidzin and genistin in soybean extract by RP HPLC was established Methods Zorbax SB C 18 column (4 6?15 mm, 5 ?m) was used Daidzein and genistein were separated with mobile phase Ⅰ∶methanol acetonitrile 0 1% phosphoric acid solution (30∶8∶62) Daidzin and genistin were separated with mobile phase Ⅱ∶methanol acetonitrie 0 1% phosphoric acid solution (16∶24∶60) The UV detection wavelength was 254 nm Results The average recoveries of daidzein, genistein, daidzin and genistin were 98 7%, 99 5%, 98 4%, 98 6% and the precision (RSD) were 1 4%, 0 9%, 0 9%, 1 7% (n=4) respectively Conclusion:
The method was accurate, rapid and with good reproducibility
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
1994
Type:
Article
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