STUDIES ON THE RELATION BETWEEN INHIBITORY EFFECT OF MYELIN MEMBRANE PROTEINS ON NEURITE OUTGROWTH AND cAMP LEVELS IN NEURONS / 解剖学报

ABSTRACT

Objective To study the relationship between the inhibitory effects that myelin membrane proteins exerted on nerve regeneration and the changes of intracellular cAMP levels in neurons. Methods To observe the effects of myelin membrane proteins extracted from central nervous system(CNS) by density centrifugation on the outgrowth of neurite of cerebella granule cells in culture and detect the changes of intracellular cAMP levels of the neurons with radioimmunoassay. Results 1^Myelin membrane proteins of CNS inhibited neurite outgrowth of cultured cerebella granule cells. 2^The cAMP level in neurons decreased in 5 minutes after contacting myelin membrane proteins and reached to the lowest level in 12 hours of contacting. Conclusion The inhibitory effects of myelin membrane proteins on the outgrowth of neurites may be related to the inhibitory factors which cause the decrease of cAMP level in neuron through the passway of signal transduction pathway. [